The Gyn Cytology High Risk (HR) test is a powerful tool for stratifying borderline Pap test results, allowing physicians to identify the 10% of patients with borderline abnormal cells. This test can help physicians determine who will progress to cervical cancer and who will not.


All women who are undergoing a routine Pap screen will benefit from the Gyn Cytology HR test. Those that can benefit the most include women who receive a test result that is borderline.

Sample Requirements
Pap test:

  • Collect specimen in ThinPrep PreservCyt vial (for cytology, FISH, and molecular).
  • Transport at room temperature in Pap transport kit.

Acceptable and preferred Pap samples should be collected from the cervix. It is best to send the sample to the lab the same day it is collected. We supply ThinPrep PreservCyt vials, which stabilize samples for up to 6 weeks at room temperature. Therefore, it is important to ensure the sample arrives within 6 weeks of being collected.

Clinical Utility

The Gyn Cytology HR test employs several markers to assess the health of the cervical cells and provide predictive information.

Ki-67Ki-67 is a protein found in cells preparing for division. A staining procedure can determine the percentage of tumor cells positive for Ki-67. The more positive cells there are, the faster they are reproducing and forming new cells.
p16p16 is a protein that suppresses tumor growth. It is often overexpressed in patients infected with high-risk HPV. The more positive cells there are for p16, the greater the chance there could be a tumor.
TERCTERC is a gene that provides directions for making an enzyme called telomerase. High telomerase activity in cancer cells enables their rapid growth and infinite proliferation potential. TERC amplification is associated with progression to CIN3 or invasive cervical cancer.
c-mycc-myc is an oncogene that oversees cell growth and division. Amplification of this gene could lead to healthy cells becoming tumor cells. In addition, the c-myc gene is the common site of HPV integration and is amplified in precancerous lesions.
CTNND2Catenin delta 2 expresses an adhesive junction protein associated with brain and eye development along with cancer formation. The protein expressed by this gene causes the decrease of E-cadherin expression, which then leads to cell movement in the presence of growth factors. Decreased expression of E-cadherin is correlated with cancer progression and metastasis.

Benefits of additional markers